标题：[Research progress on site-oriented and three-dimensional immobilization of proteins]

作者：Wang C, Feng B.

网址:PMID: 25916107

求助者：lxz77

• 求助时间：2015/6/15 11:16:18
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• 文献摘要：

In today´s post-genome era, the goals of research are geared toward understanding the meaning of information in sequenced DNA, namely, understanding the functional characteristics of proteins encoded by genomes of living beings. Protein array technologies, particularly miniaturized high-throughput platforms such as micro- or nano-fluidic chips that allow the parallel detection of thousands of proteins simultaneously, are playing increasing important roles as discovery tools in proteomics. These technologies are based on principles of molecular recognition and consist of a support surface, such as a glass slide, bead, or microtiter plate, to which an array of captured proteins is bound. However, immobilized proteins often lose their immunoactivity and suffer from low surface density, which results in inefficient signal response. In this review, we mainly provide an introduction about the research progress on site-oriented adsorption of protein at solid-liquid interfaces, especially the three-dimensional immobilization of protein, whose objective is to retain immobilized proteins in an active state at great density.

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